Effect of concentrating and exposing the bioluminescent bacteria to the non - luminescent allo - bacterial extra cellular products on their

Bioluminescence is a biochemical process occurring in many organisms. Bacterial bioluminescence has been investigated extensively that lead to many application of such knowledge. Quorum sensing in the bioluminescent bacteria is a chemical signal process to recognize the strength of its own population to start luminescence in harmony. There is a mechanism in these bacteria to recognize inter-species strength also. When there is higher number of these bacteria, the possibility and frequency of cell-cell physical contact will be high. In this study, the physical proximity was artificially enhanced between cells and the effect on luminescence in the concentrated cells in the normal culture medium and in presence of other non-bacterial cell free supernatant were investigated. The role of such physical contact in the quorum sensing in the bioluminescence is not known. Increase in the luminescence of V. fischeri when concentrated shows the presence of physical proximity facilitates the quorum sensing for their bioluminescence.